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PCR quantitative

Accueil > Plates-formes PFRN > PCR quantitative > Real-Time Quantitative PCR Support Facility

Real-Time Quantitative PCR Support Facility

Real-Time quantitative PCR (qPCR) is an accurate and sensitive method of quantifying the abundance of a target DNA sequence. This can be from genomic DNA or from cDNA resulting from the reverse-transcription of RNA.}}

A polymerase chain reaction (PCR) is performed using sequence-specific primers and a DNA-binding dye or probe that provides a fluorescent signal proportional to the amount of product (or amplicon) formed during each amplification cycle. Analysis of the amplification curves allows samples to be quantified via a standard curve, or used to calculate relative expression levels between samples (e.g. for validation of DNA arrays or confirming gene knockdown by siRNA).

Mission

The aim of the laboratory is to facilitate the effective use of the qPCR technique by the CRN2M investigators. It is also open to others researchers present on the campus. Researchers of others campus and non-university users may also take advantage of qPCR Support Facility. For pricing information please contact the scientific coordinator.

Equipment

Since July 2008 the facility provides access to two Applied Biosystems 7500 Fast Real-Time PCR Systems equipped with 96 well blocks. This platform support SybrGreen, Taqman, Molecular beacons or Scorpion reaction chemistries and is calibrated for the broadest range of dyes available : FAM™/SYBR® Green I, VIC®/JOE™, NED™/ TAMRA™/ Cy3™, ROX™/Texas Red®, and Cy5™ dyes The Applied Biosystems 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for fast cycling, the 7500 Fast delivers high-quality results in as little as 40 minutes. The facility has recently acquired a UV PCR cabinet with an HEPA filtration system for preventing contamination during the setting up of PCR reactions The users must provide the supplies and reagents for the assays.

Scientific coordinator :
  • Jean-Louis Franc, DR CNRS
  • Email : jean-louis.franc univ-amu.fr
  • Phone : +33 491 69 87 61

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