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Accueil > Bibliographie > Sequential interaction of CD4 and HIV-1 gp120 with a reconstituted membrane (...)

Sequential interaction of CD4 and HIV-1 gp120 with a (...)

Biochem Biophys Res Commun. 1998 May ;246(1):117-22
Sequential interaction of CD4 and HIV-1 gp120 with a reconstituted membrane patch of ganglioside GM3 : implications for the role of glycolipids as potential HIV-1 fusion cofactors.
Hammache D, Yahi N, Pieroni G, Ariasi F, Tamalet C, Fantini J.

The fusion of HIV-1 with CD4+ cells involves, in addition to CD4, specific cell surface molecules acting as fusion cofactors. Recently, we reported that the V3 loop of HIV-1 gp120 binds to GM3, a ganglioside abundantly expressed on CD4+ lymphocytes and macrophages. In the present study, we show that CD4 interacts with a reconstituted patch of GM3 by measuring the surface pressure with a Langmuir film balance. A biphasic increase in surface pressure is observed after the sequential addition of CD4 and gp120 under the GM3 monolayer, indicating the formation of the trimolecular complex GM3-CD4-gp120. Neutralization of gp120 with an anti-V3 antibody inhibits the secondary interaction with GM3, suggesting that the CD4-induced conformational change in gp120 allows the V3 loop to interact with GM3. In conclusion, this study supports the concept that glycolipids can function as HIV-1 fusion cofactors.

PubMed

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