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[Preparation of specific pituitary cells by centrifugal (...)

C R Seances Acad Sci III. 1981 Mar ;292(11):697-9
[Preparation of specific pituitary cells by centrifugal elutriation].
De Monti M, Enjalbert A, Duval J.

The cells of the male rat pituitary were enzymatically dispersed with trypsin and then separated by centrifugal elutriation. The commercial equipment requires minor modifications, especially the infection system. Using a gradient of flow, enriched gonadotrop cells are obtained. The results compare favourably with those obtained by sedimentation at unit gravity.


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