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Accueil > Bibliographie > Nano-liquid chromatography and mass spectrometry profiling of Tityus (...)

Nano-liquid chromatography and mass spectrometry (...)

In Advances and new technologies in toxinology (Barbier J, Benoit E, Marchot P, Mattei C, Servent D, eds), Collection Rencontres en Toxinologie, Publications de la SFET, Châtenay-Malabry, France, 2010, pp 21-23.
Nano-liquid chromatography and mass spectrometry profiling of Tityus serrulatus scorpion venom.
Pierre E. Bougis, Maya Belghazi, Marie-France Martin-Eauclaire

During the last decade, mass spectrometry (MS) has become one of the major suitable techniques for the analysis of peptide and protein components from animal venoms, not only for global mass mapping of these complex mixtures, or for structural studies on individual toxins. Here, we investigate toxic components from the scorpion Tityus serrulatus venom using the latest cutting edge advances in off-line LC/MALDI-TOF separation and peptide mass fingerprint. As a result, the nanogram range is perfectly achievable for the study of venoms.

PubMed

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