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Accueil > Bibliographie > A PQL (protein quantity loci) analysis of mature pea seed proteins (...)

A PQL (protein quantity loci) analysis of mature pea (...)

Proteomics. 2011 Mar ; [Epub ahead of print]
A PQL (protein quantity loci) analysis of mature pea seed proteins identifies loci determining seed protein composition.
Bourgeois M, Jacquin F, Cassecuelle F, Savois V, Belghazi M, Aubert G, Quillien L, Huart M, Marget P, Burstin J.

Legume seeds are a major source of dietary proteins for humans and animals. Deciphering the genetic control of their accumulation is thus of primary significance towards their improvement. At first, we analysed the genetic variability of the pea seed proteome of three genotypes over 3 years of cultivation. This revealed that seed protein composition variability was under predominant genetic control, with as much as 60% of the spots varying quantitatively among the three genotypes. Then, by combining proteomic and quantitative trait loci (QTL) mapping approaches, we uncovered the genetic architecture of seed proteome variability. Protein quantity loci (PQL) were searched for 525 spots detected on 2-D gels obtained for 157 recombinant inbred lines. Most protein quantity loci mapped in clusters, suggesting that the accumulation of the major storage protein families was under the control of a limited number of loci. While convicilin accumulation was mainly under the control of cis-regulatory regions, vicilins and legumins were controlled by both cis- and trans-regulatory regions. Some loci controlled both seed protein composition and protein content and a locus on LGIIa appears to be a major regulator of protein composition and of protein in vitro digestibility.


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