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Accueil > Bibliographie > A new system for expressing recombinant animal toxins in E. coli (...)

A new system for expressing recombinant animal toxins (...)

In Advances and new technologies in toxinology (Barbier J, Benoit E, Marchot P, Mattei C, Servent D, eds), Collection Rencontres en Toxinologie, Publications de la SFET, Châtenay-Malabry, France, 2010, pp 149-152.
A new system for expressing recombinant animal toxins in E. coli ?
Badreddine Douzi, Arie Geerlof, Hervé Darbon, Nicolas Gilles, Pascale Marchot, Renaud Vincentelli

Recombinant expression in bacteria remains the most “high yield, low cost” means for production of disulfide-rich peptides. Unfortunately, in most cases a long process of renaturation is necessary to obtain properly folded and fully active toxins. Here we describe a method that we have set up and successfully validated for the production of active toxins from animal venoms in E. coli.

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