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Accueil > Agenda > Les séminaires Jean Roche > Imaging development and plasticity in the mouse visual system.

Imaging development and plasticity in the mouse visual (...)

Lundi 13 juin 2005, 11h, salle Lissitzky.


1 : Curr Biol. 2005 Mar 29 ;15(6):R205-8.

Visual cortex : two-photon excitement.

Hubener M, Bonhoeffer T.

Max-Planck-Institut fur Neurobiologie, Martinsried, Germany. mark

Current in vivo methods for imaging the visual cortex lack the ability to map response properties at the level of single cells. A new technique using two-photon imaging of calcium signals has now overcome this limitation.

2 : Curr Biol. 2003 Sep 30 ;13(19):R778-80.

Brain mapping : new wave optical imaging.

Mrsic-Flogel T, Hubener M, Bonhoeffer T.

Max-Planck-Institut fur Neurobiologie, Am Klopferspitz 18A, D-82152 Martinsried, Germany. flogel

Optical imaging of intrinsic signals is widely used for high-resolution brain mapping in various animal species. A new approach using continuous data acquisition and Fourier decomposition of the signal allows for much faster mapping, opening up the possibility of applying this method to new experimental questions.

3 : Curr Opin Neurobiol. 2003 Aug ;13(4):413-20.

Mouse visual cortex.

Hubener M.

Max-Planck-Institut fur Neurobiologie, Am Klopferspitz 18A, D-82152 Martinsried, Germany. mark

Neurons in mouse visual cortex have diverse receptive field properties and they respond selectively to specific features of visual stimuli. Owing to the lateral position of the eyes, only about a third of the visual cortex receives input from both eyes, but many cells in this region are binocular. Similar to higher mammals, closing one eye during a critical period shifts the responses of cells, such that they are better driven by the non-deprived eye. In this review I illustrate how the combination of transgenic mouse technology with single cell recording and modern imaging techniques might lead to a further understanding of the mechanisms that underlie the development, plasticity, and function of the mammalian visual cortex.

4 : Curr Biol. 2002 Aug 20 ;12(16):R547-9.

Visual cortex : suppression by depression ?

Mrsic-Flogel T, Hubener M.

Max-Planck-Institut fur Neurobiologie, Am Klopferspitz 18A, D-82152, Martinsried, Germany.

The response of a neuron in the visual cortex to an oriented light bar is strongly reduced by concurrent presentation of a stimulus with a different orientation. New data suggest this ’cross-orientation suppression’ is caused, not by intracortical inhibition, but by rapid depression of thalamocortical synapses.

5 : Neural Comput. 2002 Sep ;14(9):2053-6. Comment on : Neural Comput. 2002 Jul ;14(7):1545-60.

Reply to Carreira-Perpinan and Goodhill. Are visual cortex maps optimized for coverage ?

Swindale NV, Shoham D, Grinvald A, Bonhoeffer T, Hubener M.

Department of Ophthalmology, University of British Columbia, Vancouver, B.C., V5Z 3N9. swindale

6 : J Neurosci. 2002 Aug 1 ;22(15):6549-59.

Mapping retinotopic structure in mouse visual cortex with optical imaging.

Schuett S, Bonhoeffer T, Hubener M.

Max-Planck-Institut fur Neurobiologie, D-82152 Martinsried, Germany.

We have used optical imaging of intrinsic signals to visualize the retinotopic organization of mouse visual cortex. The functionally determined position, size, and shape of area 17 corresponded precisely to the location of this area as seen in stained cortical sections. The retinotopic map, which was confirmed with electrophysiological recordings, exhibited very low inter-animal variability, thus allowing averaging of maps across animals. Patches of activity in area 17 were often encircled by regions in which the intrinsic signal dropped below baseline, suggesting the presence of strong surround inhibition. Single-unit recordings revealed that this decrease of the intrinsic signal indeed correlated with a drop of neuronal firing rate below baseline. The averaged maps also greatly facilitated the identification of extrastriate visual activity, pointing to at least four extrastriate visual areas in the mouse. We conclude that optical imaging is ideally suited to visualize retinotopic maps in mice, thus making this a powerful technique for the analysis of map structure in transgenic animals.

7 : Neuron. 2001 Oct 25 ;32(2):325-37.

Pairing-induced changes of orientation maps in cat visual cortex.

Schuett S, Bonhoeffer T, Hubener M.

Max-Planck-Institut fur Neurobiologie, 82152, Martinsried, Germany.

We have studied the precise temporal requirements for plasticity of orientation preference maps in kitten visual cortex. Pairing a brief visual stimulus with electrical stimulation in the cortex, we found that the relative timing determines the direction of plasticity : a shift in orientation preference toward the paired orientation occurs if the cortex is activated first visually and then electrically ; the cortical response to the paired orientation is diminished if the sequence of visual and electrical activation is reversed. We furthermore show that pinwheel centers are less affected by the pairing than the pinwheel surround. Thus, plasticity is not uniformly distributed across the cortex, and, most importantly, the same spike time-dependent learning rules that have been found in single-cell in vitro studies are also valid on the level of cortical maps.

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